Visual diagram of a biophysical model of increasing the affinity of an antibody for an antigen
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly by plasma cells that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses. Antibody development is the procedure of creating and characterizing an antibody. Their origin is mammalian B-cells and they are present in blood circulation and lymph nodes. IgY polyclonal and monoclonal antibodies.Antibody development is a multi-faceted process and an experiment in itself. To assist in generating an immune response towards your antigen and ensure
STEP ONE
1.Determination of three-dimensional complex of the target protein with antibody flexible chain, which is subject to further modification. This should be at least one file with the extension of the PDB obtained by the method of X-ray diffraction analysis.
2.To control the received data, you can choose either of two options:
- you can use the additional structure of the PDB.
-take advantage of previously available data on the mutations performed, alanine scanning of one of the participants of the antibody-antigen complex
3.Our experts check files, adapt them for computational manipulations.
STEP TWO
4. Our specialists perform the necessary calculations: obtain data, numerically calculate the results in the form of graphs and diagrams, determine key amino acid residues, interaction energies, changes in affinity and stability, change in entropy for each replacement of the amino acid residue in the flexible chain of immunoglobulin.
5. Performing a verification series of calculations in accordance with paragraph 2
1.Determination of three-dimensional complex of the target protein with antibody flexible chain, which is subject to further modification. This should be at least one file with the extension of the PDB obtained by the method of X-ray diffraction analysis.
2.To control the received data, you can choose either of two options:
- you can use the additional structure of the PDB.
-take advantage of previously available data on the mutations performed, alanine scanning of one of the participants of the antibody-antigen complex
3.Our experts check files, adapt them for computational manipulations.
STEP TWO
4. Our specialists perform the necessary calculations: obtain data, numerically calculate the results in the form of graphs and diagrams, determine key amino acid residues, interaction energies, changes in affinity and stability, change in entropy for each replacement of the amino acid residue in the flexible chain of immunoglobulin.
5. Performing a verification series of calculations in accordance with paragraph 2
STEP THREE
6. Analysis of the array of data obtained, selection of the most suitable replacements, report generation.
7.Sending the received data to the customer.
6. Analysis of the array of data obtained, selection of the most suitable replacements, report generation.
7.Sending the received data to the customer.
Antibody development is the procedure of creating and characterizing an antibody.
Ordering process and execution
